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dilute primary antibody  (Proteintech)


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    Structured Review

    Proteintech dilute primary antibody
    Dilute Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 593 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diluted+primary+antibody/GFAP+Polyclonal+antibody/pm40987409-156-6-10
    Average 96 stars, based on 593 article reviews
    dilute primary antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Microsecond pulsed electric fields induce myocardial ablation by secondary mitochondrial damage and cell death mechanisms.
    Article Snippet: TWEEN-20:Hushi) at room temperature for 1 h, incubated overnight with the diluted primary antibody (Proteintech) at 4 °C, and washed thrice with TBST.

    Article Title: CSNK1E is involved in TGF-β1 induced epithelial mesenchymal transformationas and related to melanoma immune heterogeneity
    Article Snippet: The diluted primary antibody (Proteintech, United States) was then introduced to the wells, and the 12-well plate was incubated overnight at 4°C on a shaker.

    Article Title: Macrophage-targeted, ROS-removing and H2S-releasing nanomicelle encapsulated in hydrogel microspheres for the treatment of osteoarthritis
    Article Snippet: The progression and severity of osteoarthritis (OA) are significantly associated with intra-articular synovitis, with the imbalance of M1/M2 macrophages serving as a critical driving factor in the pathogenesis of synovitis.. In this study, we developed macrophage-targeted nanomicelles capable of scavenging reactive oxygen species (ROS) and releasing hydrogen sulfide (H2S).. These nanomicelles were encapsulated within injectable methacrylate gelatin (GelMA) porous hydrogel microspheres, thereby creating a new H2S gas therapy platform for the treatment of osteoarthritis.

    Flow Cytometry:

    Article Title: Microsecond pulsed electric fields induce myocardial ablation by secondary mitochondrial damage and cell death mechanisms.
    Article Snippet: TWEEN-20:Hushi) at room temperature for 1 h, incubated overnight with the diluted primary antibody (Proteintech) at 4 °C, and washed thrice with TBST.

    Article Title: CSNK1E is involved in TGF-β1 induced epithelial mesenchymal transformationas and related to melanoma immune heterogeneity
    Article Snippet: The diluted primary antibody (Proteintech, United States) was then introduced to the wells, and the 12-well plate was incubated overnight at 4°C on a shaker.

    Article Title: Macrophage-targeted, ROS-removing and H2S-releasing nanomicelle encapsulated in hydrogel microspheres for the treatment of osteoarthritis
    Article Snippet: The progression and severity of osteoarthritis (OA) are significantly associated with intra-articular synovitis, with the imbalance of M1/M2 macrophages serving as a critical driving factor in the pathogenesis of synovitis.. In this study, we developed macrophage-targeted nanomicelles capable of scavenging reactive oxygen species (ROS) and releasing hydrogen sulfide (H2S).. These nanomicelles were encapsulated within injectable methacrylate gelatin (GelMA) porous hydrogel microspheres, thereby creating a new H2S gas therapy platform for the treatment of osteoarthritis.



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    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) <t>Immunostaining</t> images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.
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    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) <t>Immunostaining</t> images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.
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    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) <t>Immunostaining</t> images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.
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    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) <t>Immunostaining</t> images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.
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    Image Search Results


    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

    Journal: iScience

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

    doi: 10.1016/j.isci.2025.114190

    Figure Lengend Snippet: The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

    Techniques: Immunostaining, Fluorescence

    The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

    Journal: iScience

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

    doi: 10.1016/j.isci.2025.114190

    Figure Lengend Snippet: The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

    Techniques: Immunostaining, Staining